Effect of salicylic acid (SA) and gibberellic acid (GA 3 ) pre-soaking on seed germination of stevia (Stevia rebaudiana) under salt stress

2012 
The role of salicylic acid (SA) and gibberellic acid (GA3) pre-soaking were examined on stevia (Stevia rebaudiana) seed germination under NaCl or NaHCO3 stress. A set of seeds was pre-soaked in H2O, another set of seeds in 0, 0.1, 0.5, 1.0, 5.0 and 10.0 mM SA and another set in 200 ppm GA3 for 24 h. Seeds were then placed in Petri-dishes with 5 ml of 0, 50, 100, 200 and 300 mM NaCl or NaHCO3. Under the highest salinity condition no germination was observed. Seed germination was 65% in H2O, 0, 5 and 1 mM SA, 33% in 5 mM SA, 12, 5% in 10 mM SA, 90% in 200 ppm GA3 44% in 50 mM NaCl, 14% in 100 mM NaCl, 40% in 50 mM NaHCO3, 11% in 100 mM NaHCO3. The germination velocity was 10.50 in H2O, 6.50 in 1 mM SA, 15.00 in 200 ppm GA3, 7.00 in 50 mM NaCl, 1.50 in 100 mM NaCl, 5.00 in 50 mM NaHCO3, 1.00 in 100 mM NaHCO3. Seed germination and velocity in NaCl was higher than the corresponding ones in NaHCO3. By pre-soaking in 0.5, 1.0 mM SA the seed germination was as much as in H2O, while in 5.0, 10.0 mM SA they were less than in H2O. By pre-soaking in all SA concentrations, the 50 or 100 mM NaCl and also in NaHCO3, this resulted in the reduction of germination in all combinations, when compared to their respective one in only 50 or 100 mM NaCl. In all SA concentrations the velocity was lower than the corresponding one in H2O. Highest velocity was observed in 200 ppm GA3.
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