Searching for relief: Drosophila melanogaster navigation in a virtual bitter maze.

Abstract Animals use relief-based place learning to pinpoint a specific location where noxious stimuli are diminished or abolished. Here we show how the optogenetically-induced activation of bitter-sensing neurons in Drosophila melanogaster elicits pain-like behavioural responses and stimulates the search for a place where this activation is relieved. Under this “virtual” stimulation paradigm it would be feasible to test relief learning several times throughout an animal's lifespan, without the potentially damaging effects which may result from the repeated administration of “real” heat or electrical shock. Furthermore, virtual bitter taste could be used in place of virtual pain stimulation to guide conditioned place preference and study learning processes. We also propose that spatially-specific reduction of locomotor velocity may provide immediate evidence of relief-based place learning and spatial memory.