Cloning the Major Epitope Domain of gE of Pseudorabies Virus EaStrain and Its Expression In E.coli

A0 .8kb DNA fragmentencoding the majorepitope domain of glycoprotein g E of pseu- dorabies virus Ea strain was amplified by PCR technique and cloned into p Bluescript SK+ .The se- quence of the fragmentwas obtained by Sanger′s sequencing technique.Then,the fragmentwas insert- ed into downstream of the T7promoter of an expression vector,p ET-1 7b,to yield the recombinant plasmid p ETE80 4.After induction by IPTG,a high expression of fusion protein was obtained,SDS- PAGE analysis and Western blotting showed that the fusion protein was measured of3 80 0 0 specific to antisera against PRV Ea strain than against Bartha strain in Western blotting.This indicated that the expression products could be useful for the differentiation of natural infection from vaccinated herds.