Purification method of penicillin and cephalosporin

The invention discloses a method for purifying penicillin and cephalosporin, which comprises the following steps that: the penicillin or the cephalosporin is dissolved into water, the mixture is loaded to a chromatographic column filled by a Toyopearl HW-40 filler, the water or an electrolyte solution is used to elute an eluent, and the purified penicillin or the cephalosporin is obtained after the separation. The method separates macromolecular impurities in the penicillin and the cephalosporin by a chromatography, and a filler adopted by the method is Toyopearl HW-40; and the filler can produce adsorption effect on the penicillin and the cephalosporin when taking a water solution as a mobile phase, so the separation effect of the macromolecular impurities in the penicillin and the cephalosporin is improved greatly.