Development of anti-phenylurea antibody purification techniques for improved environmental applications

The non-uniform affinity of polyclonal antibodies is a major problem when these antibodies are used in immunochemical-based environmental applications. A specific affinity chromatography procedure was developed to extract selectively anti-isoproturon antibodies from a crude polyclonal antiserum. These antibodies were fully characterized and helped to improve the performances of immunoaffinity extraction and an indirect enzyme immunoassay.