Modulation of inflammatory mediators by coumarinolignoids from Cleome viscosa in female swiss albino mice

The effect of coumarinolignoid cleomiscosins A, B and C isolated from the plant Cleome viscosa on inflammatory mediators were studied in female swiss albino mice. A mixture of coumarinolignoid A, B, and C at 10, 30 and 100 mg/kg body weight once a day for 14 consecutive days were administered orally to the mice. Pro–inflammatory mediators such as IL–6, TNF–α and nitric oxide were estimated from culture supernatant obtained from peritoneal macrophages stimulated by LPS and anti-inflammatory mediator IL–4 was estimated from culture supernatant obtained from spleenocytes stimulated by Con-A. For further confirmation, expressions of inflammatory mediators from serum and mortality rate were studied in LPS–induced toxicity model in mice. The expression of Pro–inflammatory mediators was significantly (P <0.05) decreased in coumarinolignoids treatment group in dose dependent manner, whereas the anti–inflammatory mediator expression was significantly increased in coumarinolignoids at 10 mg/kg treatment. Mortality rate was also significantly reduced in treatment group in LPS–induced toxicity model. The result of this study concluded that the oral administration of coumarinolignoids inhibited the pro–inflammatory mediators and enhances the production of anti–inflammatory mediator in dose dependent manner.