Expression of key enzymes in glucose metabolism associated with oxygen condition in bifidobacterium longum BBMN68

Bifidobacteria is a kind of anaerobic bacteria,the ability of oxygen tolerance is one of the most important properties of Bifidobacteria. To investigate the genes expression of glucose of Bifidobacterium Longum BBMN68 which incubated in different oxygen condition,a semi-quantitative RT-PCR method was used to detect the key enzyme genes expression in 4% oxygen condition. The results indicated that the growth of Bifidobacterium Longum BBMN68 had been restrained by the oxygen stress,the mRNA level of the enzymes was depressed under 4% oxygen condition for 30 minutes,however,the mRNA level of xfp and gap had recovered under 4% oxygen condition for 60 minutes and more. Maybe it had some relationship between glucose metabolism enzymes and the growth ability of Bifidobacterium Longum BBMN68 in oxygen condition.