Chloroquine prevents acute kidney injury induced by lipopolysaccharide in rats via inhibition of inflammatory factors

Purpose: To investigate the role of chloroquine (CQ) in lipopolysaccharide (LPS)-induced renal injury in rats. Methods: Rats were assigned to one of four groups ( n = 10). Control group was only given saline solution, whereas the model control, LPS + CQ, and LPS + yohimbine (YOH) + CQ groups were administered LPS intraperitoneally. At the end of the study, blood urea nitrogen (BUN) and creatinine (Cr) levels were determined. Results: CQ treatment significantly decreased the blood concentrations of tissue necrosis factor alpha (TNF-α), interleukin-6 (IL-6), IL-18, BUN, and Cr in the model control rats. There were also significant decreases in the levels of high mobility group protein 1 and kidney injury molecule-1 in the renal injury rats compared to the model control group. However, the inhibitory effects of CQ in the LPS-treated rats were blocked by treatment with YOH, an α-2-adrenergic receptor antagonist. Conclusions: Treatment with CQ attenuates LPS-induced renal injury by inhibiting inflammatory response. Keywords: Creatinine, Chloroquine, Inflammatory reactions, Kidney injury, Lipopolysaccharide