Effect of Jingyuankang Capsule on Expression of Apoptosis-related Genes in Bone Barrow Cells of Model Mice of Chemotherapy-induced Myelosuppression

Objective:To observe the effect of Jingyuankang capsule on the expression of apoptosis-related genes Caspase-3,Fas and FasL mRNA in bone marrow cells and make a study of the mechanism of the medicine.Method:Sixty mice were randomized into 5 groups:control group,model group,positive control group(Leucogen,32 mg·kg-1),Zhenqi Fuzheng group(3 g·kg-1),Jingyuankang group(4 g·kg-1).The model was induced by ip cyclophosphamide(100 mg·kg-1) for 3 days.Mean while the above treatments were given for 7 days.The expression of Caspase-3,Fas and FasL mRNA in bone marrow cells of model mice with myelosuppression was measured by real-time fluorescence quantitative PCR.Result:The Jingyuankang group had the lowest expression of Caspase-3,Fas and FasL mRNA,compared with that of the model group,the difference was significant;while compared with the control group,there was no significant differences.Conclusion:These results confirmed preliminarily that Jingyuankang capsule could decrease the expression of apoptosis-related genes Caspase-3,Fas and FasL,through affecting the apoptosis-related pathway to prevent the blood cells from apoptosis caused by chemotherapy.This might be an important mechanism of Jingyuankang capsule treating the chemotherapy-induced myelosuppression.