Pleural and serum DNA in patients with cancer and pleural effusion: A quantitative diagnostic tool

9519 Background: Pleural effusion is a frequent diagnostic problem in patients with malignancy and may have therapeutic and prognostic impact Methods: Measurement of DNA from pleural liquid (Pl) and peripheral blood (Pb) in patients (p) with pleural effusion (PE) and malignant neoplasm (study group A) and compare the obtained values in p with PE without malignancy (control group B). Group A of 44 samples in p with malignant neoplasm and PE; Group B 19 p with PE without malignancy. DNA were extracted and quantified in 2 simultaneous samples of Pl and Pb collected after 15 min centrifugation at 3000 rpm, aliquoted in 1 ml sterile vials and frozen until DNA extraction. DNA was extracted using DNA blood Qiagen kit. The amount of plasma and pleural DNA was determined by RT quantitative PCR amplification of the human RNase P, a single-copy gene encoding the RNA moiety for the RNase P enzyme. Mann-Whitney test compare the measured values of DNA from Pl and Pb. ROC curve performed to evaluate the diagnostic capac...