Comparative study on Angelica sinensis endophytic bacteria and the rhizospheric soil microbial diversity in three main producing areas of Gansu

In order to analyze the taxonomic community composition of endophytic bacteria in Angelica sinensis (Danggui), as well as the distribution of microbial bacteria in the rhizospheric soil, Danggui and the rhizospheric soil samples were collected from Meichuan town Min county (MX, S-MX), Huichuan town Weiyuan county (HC, S-HC) and Dongjiang town Wudu county (WD, S-WD) in Gansu. The microbiota in Danggui and the rhizospheric soil samples were collected, then the 16S ribosomal DNA (16S rDNA) were extracted and amplified using PCR, finally sequenced by Illumina Miseq High-throughput sequencing technology. The Alpha diversities of Danggui and the rhizospheric soil samples show: The Chao1, ACE and Shannon indices of samples were in order of MX>WD>HC, S-MX>S-WD>S-HC; 4 dominant shared phylum, Proteobacteria, Cyanobacteria, Bacteroidetes, Firmicutes were observed in MX, HC, and WD samples respectively, 5 major shared phylum observed were Proteobacteria, Actinobacteria, Planctomycetes, Bacteroidetes, Acidobacteria in S-MX, S-HC, S-WD samples respectively. Venn diagrams suggest that some of the endophytic bacteria in Danggui are derived from the rhizospheric soil, and the microbial content is positively correlated with the quality of Danggui. To some extent, there may be a certain relationship between the quality of Danggui and its endophytes and rhizospheric soil microorganisms.