Quantities of receptor molecules for colony stimulating factors on leukocytes in measles.

We analyzed the comparative amounts of granulocyte-colony stimulating factor (G-CSFr) and granulocyte macrophage CSF (GM-CSFr) receptors expressed on neutrophils and monocytes in measles patients to investigate the role of these CSFrs in the development of leukopenia including neutropenia and monocytopenia in measles. EDTA-anticoagulated peripheral blood of 19 measles patients, 10 children with other infections showing leukopenia and 16 children with normal complete blood cell counts (CBC)s were analyzed using flow cytometry and QuantiBRITE. The leukocyte ( vs. 9900 + 2680/uL, p=0.000), neutrophil ( vs. , p=0.024) and the lymphocyte counts of measles patients ( vs. , p= 0.006) were lower than in the normal controls. The neutrophils of measles patients expressed similar amounts of G-CSFr as normal children (, p= 0.564) and leukopenic patients (, p=0.713), but lower levels of GM-CSFr than normal children (, p=0.000) and leukopenic patients (, p=0.002). The monocytes of measles patients expressed similar amounts of G-CSFr and GM-CSFr as normal children ( & 4125 (2645, p > 0.05) but less than leukopenic patients $(1454{\pm}398\;&\;5388{\pm}806,\;p>0.05)$. The neutrophil and monocyte counts of measles patients did not correlate with the amount of G-CSFr or GM-CSFr expressed on neutrophils or monocytes (p > 0.05), but in the normal children, the monocyte count correlated with the levels of GM-CSFr on monocytes (r=0.951, p=0.049). In conclusion, neutropenia is one of the more important characteristics of measles patients, which could be due to the decreased GM-CSFr expression on neutrophils. However, the monocytopenia found in measles patients is not due to the decreased expression of CSFr on the monocytes.