Characterization of the soybean gene GmENOD40-2

The GmENOD40-2 gene was isolated from a soybean genomic library and the nucleotide sequence of a 3 kb EcoRI/HindIII fragment was determined. A 1.7 kb fragment of the GmENOD40-2 promoter region was used in a transcriptional fusion construct with GUS. In transgenic Vicia nodules containing this construct, β-glucuronidase activity was detected in the pericycle of the nodule vascular bundles as well as in the root pericycle at the attachment point of the nodule