Hydrogen and carbon dioxide mixed culture fermentation in a hollow-fiber membrane biofilm reactor at 25 °C

Abstract There have been no reports of H 2 and CO 2 mixed-culture fermentation (MCF) at 25 °C in a hollow-fiber membrane biofilm reactor (HfMBR). In this study, H 2 and CO 2 MCF were conducted in an HfMBR at 25 °C producing metabolites including acetate, ethanol, butyrate, and caproate. Compared to pure culture fermentation (i.e., Clostridium carboxidivorans P7), the MCF in HfMBR at 25 °C produced a higher concentration of caproate in this study (3.4 g/L in batch 1 and 5.7 g/L in batch 2). The dominant genera were Clostridium_sensu_stricto_12 and Prevotella_7 . The caproate was more likely formed from the pathway of acetate and ethanol rather than via butyrate and ethanol. Since caproate is more valuable than acetate and low temperature fermentation consumes less energy, this process of H 2 and CO 2 MCF at 25 °C is appropriate for industrial application.