SH2 mutants of c-src that are host dependent for transformation are trans-dominant inhibitors of mouse cell transformation by activated c-src.

The c-src gene encodes a membrane-associated protein-tyrosine kinase, pp60 c-src , whose substrates and regulators have not been identified. In an effort to obtain mutants that might assist the search for proteins that interact with pp60 c-src , we have generated by site-directed mutagenesis two alleles of chicken c-src that are host dependent for transformation and inhibit transformation of mouse cells by activated c-src in a trans-dominant manner