AVALIAÇÃO DA MULTIPLICAÇÃO DOS ESPOROS DE FUNGOS MICORRÍZICOS ARBUSCULARES NA SAVANA DE ALTER DO CHÃO, PARÁ

The savannah biome has edaphoclimatic conditions conducive to the occurrence of Arbuscular Mycorrhizal Fungi (AMF), which associated with plants, become biotechnological tools of underestimated potential. One of the reasons is the difficulty in producing and spreading infectious propagules in a significant amount to develop studies under controlled conditions. The objective of this work was to analyze the multiplication of spores from the AMF from the Savannah of Alter do Chao, Western Para, and the aspects of symbiosis in Brachiaria decumbens Stapf. as a hostess. The experiment was conducted under controlled conditions at the Laboratory of Plant Physiology and Plant Growth at the Federal University of Western Para from February to October 2019. Two types of soils (commercial substrate and dystrophic yellow latosol) were tested at different time periods (20, 36, 65 and 129 days). For this, composite samples containing spores of native AMF were collected in the study area, of which 100 g were homogenized to 850 g of the tested soils, separately, in 1 kg pots. The experiment was carried out under laboratory conditions and the design was completely randomized, in a 2 × 4 factorial scheme (multiplication time × soil types) with four replications. The result of the spore density of the native FMA and the percentage of infection were submitted to Analysis of Variance and the means were compared by the Scott Knott test with p <0,05 of significance. The commercial substrate provided the best results in spore production and percentage of infection in roots of B. decumbens at 20 days of inoculation. For the multiplication and production of infectious propagules of the AMF of the Savannah of Alter do Chao, the use of the commercial substrate cultivated for a period of 20 days is indicated.