胰島素、地塞米松、胰島素樣生長因子－1(IGF-1)對3T3-L1脂肪細胞脂肪水孔蛋白表達的調節作用

Objective: To investigated the effect of hormone such as insulin, IGF-1 and dexamethasone on expression of AQPap in 3T3-L1 adipocytes, in order to know the role of AQPap in obesity and diabetes mellitus. Methods: 3T3-L1 cells on day 9 after differentiation were incubated with 10^(-6), l0^(-7), 10^(-8) and l0^(-9)mol/L insulin for 6 h, or incubated in DMEM with 10^(-8) mol/L insulin for 0, 3, 6 h for the experiment on time course, and total cellular RNA were extracted and used for RT-PCR. Such cells were also stimulated by IGF-1 (10^(-7), l0^(-8), l0^(-9), 10^(-10)mol/L), dexamethasone (l0^(-6), l0^(-7), l0^(-8)mol/L) or insulin(l0^(-6)mol/L) + dexamethasone (10^(-6)mol/L) respectively, and total RNA was isolated for RT-PCR. Results: Treatment with 10^(-9)mol/L insulin suppressed AQPap mRNA expression in differentiated 3T3-L1 adipocytes (P＜0.05), and the results showed that the suppression of insulin on the expression of AQPap mRNA was dose-dependent and time-dependent in 3T3-Ll cells. IGF-l could also decrease AQPap mRNA expression in differentiated 3T3-Ll adipocytes in l0 mol/L, but the suppression was weak compared to insulin with the same concentration. Desamethasone had no effect on the expression of AQPap mRNA in 3T3-L1 cells, but it could decrease the inhibition of insulin on the AQPap gene expression. Conclusion: Insulin is a negative regulator of AQPap expression. The inhibition of IGF-l on AQPap expression is less than that of insulin at the same stimulation concentration. Dexamethasone can antagonize the negative regulation of insulin on AQPap expression.