Glycosylation of human plasma lipoproteins reveals a high level of diversity, which directly impacts their functional properties

Abstract Aims Human plasma lipoproteins are known to contain various glycan structures whose composition and functional importance are starting to be recognized. We assessed N -glycosylation of human plasma HDL and LDL and the role of their glycomes in cellular cholesterol metabolism. Methods N -glycomic profiles of native and neuraminidase-treated HDL and LDL were obtained using HILIC-UHPLC-FLD. Relative abundance of the individual chromatographic peaks was quantitatively expressed as a percentage of total integrated area and N -glycan structures present in each peak were elucidated by MALDI-TOF MS. The capacity of HDL to mediate cellular efflux of cholesterol and the capacity of LDL to induce cellular accumulation of cholesteryl esters were evaluated in THP-1 cells. Results HILIC-UHPLC-FLD analysis of HDL and LDL N -glycans released by PNGase F resulted in 22 and 18 distinct chromatographic peaks, respectively. The majority of N -glycans present in HDL (~70%) and LDL (~60%) were sialylated with one or two sialic acid residues. The most abundant N -glycan structure in both HDL and LDL was a complex type biantennary N -glycan with one sialic acid (A2G2S1). Relative abundances of several N -glycan structures were dramatically altered by the neuraminidase treatment, which selectively removed sialic acid residues. Native HDL displayed significantly greater efficacy in removing cellular cholesterol from THP-1 cells as compared to desialylated HDL (p  Conclusions N -glycome of human plasma lipoproteins reveals a high level of diversity, which directly impacts functional properties of the lipoproteins.