Monitoring of NADH Together with Other Tissue Physiological Parameters

In the previous chapters, it was shown that monitoring of mitochondrial NADH is critical in understanding tissue oxygen balance. Nevertheless, because of the complexity of the physiological and biochemical processes, it is unavoidable and necessary to monitor as many parameters as possible at the tissue level together with mitochondrial NADH. During the past 40 years, we adopted this concept, and various techniques were developed and applied to different models of experimental animals exposed to many types of perturbations. The development of the technology included various sensors or probes that provide information on energy supply and demand in the tissue as described in this chapter. The various probes were organized in a compact multi-probe assembly that was located on the surface of the tissue without any visible damage. The various systems that were developed are presented in chronological order.