Effects of hyperbaric oxygen on the tumor growth of human pharyngeal squamous cancer in mouse model

Objective To explore the effect of hyperbaric oxygen (HBO) on the tumor growth of human pharyngeal cancer in mouse model.Methods Thirty-two male Balb/c mice were randomly divided into 4 groups:group A or the control group (sacrificed 5 days after tumor loading),group B (sacrificed 10 days after tumor loading),group C (sacrificed 5 days after tumor loading and HBO exposure),and group D (sacrificed 10 days after tumor loading and HBO exposure),each consisting of 8 animals.Following vaccination with the digested pharyngeal cancer Fadu cells in logarithmic phase,the tumor-loaded mice were respectively exposed toatmospheric air and HBO.Then,tumor volume,weight,histopathology and immunohistochemistry were detected for the animals of each group.Results Histopathology of the tumor-loaded mice in each group displayed low-differentiated squamous cell carcinoma.For the animals in group C,tumor mass inhibition rate was 20.13％,and volume inhibition rate was 20.54％.The expression level of fibroblast activation protein-alpha (FAPα) was obviously inferior to that of group A (P ＜ 0.05).For the animals in group D,tumor mass inhibition rate was 13.65％,and volume inhibition rate was 10.30％ and the expression level of FAPα was obviously inferior to that of group B (P ＜ 0.05).Conclusions HBO seemed to have certain effect on the inhibition of tumor growth of human pharyngeal cancer in mice. Key words: Hyperbaric oxygen; Pharyngeal cancer; Mouse,naked; Tumor inhibition