Role for angiotensin II in an overt functional proteinuria

Role for angiotensin II in an overt functional proteinuria. A partial renal vein constriction (RVC) was induced acutely in Munich–Wistar rats. RVC caused a marked reduction in glomerular plasma flow rate, and rises in glomerular transcapillary hydraulic pressure difference and efferent arteriolar resistance. These changes were associated with a marked increase in urinary protein excretion, on average from a baseline level of 8 to ≍ 120mg/24hrs per kidney. Infusion of saralasin, an angiotensin II (AII) antagonist, largely normalized these indices, including urinary protein excretion (to ≍ 35mg/24hrs per kidney), despite continued RVC. In separate rats, fractional clearances of neutral [ 125 I]dextrans (molecular radii=18–60 Å) (C DEX /C IN ) were measured. RVC caused a significant increase in C DEX /C IN for large dextrans (≥44Å), but not small dextrans (≤42Å). Saralasin infusion led to a partial return toward baseline values of C DEX /C IN for the large dextrans. On the basis of the heteroporous membrane theory for glomerular filtration, the glomerular sieving defect during RVC was attributed to an increase in the relative fluid flux through a group of large non-selective pores. A marked alteration in glomerular microcirculatory pattern induced by enhanced action of endogenous AII in turn seemed to account largely, although not entirely, for the impairment of glomerular size–selectivity during RVC.