Resveratrol reduces the frequency of radiation-induced chromosome aberrations in mouse bone marrow cells

A158 The purpose of this study was to investigate if resveratrol ( trans -3,5,4’-trihydroxystilbene) could reduce the frequency of radiation-induced chromosome aberrations in mouse bone marrow cells. Resveratrol has previously been shown to possess antioxidant properties, contribute to cell cycle arrest, and induce apoptosis in damaged cells. We evaluated the ability of resveratrol to reduce the frequency of chromosome aberrations in bone marrow of 10 week old, male CBA/CaJ mice. Mice were divided into groups of 10 mice each for the following treatments: 1) no treatment (NoTx), 2) resveratrol only (RES), 3) radiation only (RAD), 4) resveratrol initiated before radiation (RES>RAD), and 5) radiation prior to resveratrol initiation (RAD>RES). All irradiated mice received one 3-Gy dose of whole body gamma-radiation. The RES>RAD group received resveratrol (100 mg/kg) daily via gavage for three days prior to radiation exposure, with the third resveratrol dose administered 30 minutes before irradiation. Resveratrol administration continued mixed in the drinking water at a daily dose of 100 mg/kg. Bone marrow was collected at one and 30 days post-irradiation. For mice in the RAD>RES group, resveratrol (100 mg/kg) was administered either a) as a single gavage dose two hours after radiation exposure, b) initiated by gavage two hours post-irradiation and continued in the drinking water, or c) started two days after radiation in the drinking water. Bone marrow was collected at one, seven, and 30 days post-irradiation. For each time point, collected bone marrow was processed for cytogenetic analyses. Giemsa stained slides were blinded and 25 metaphase cells from each mouse were scored for chromosome aberrations. Initiation of resveratrol either before or after whole body irradiation had a significant impact on the number of chromosome aberrations observed at all time points. Mean total chromosome aberration frequencies were found to vary by treatment group and time point. When resveratrol was given pre-irradiation (RES>RAD), the Kruskal-Wallis ANOVA p-value for the comparison of the four treatment groups was RAD vs. RAD groups, and RES>RAD vs. RES groups. Initiation of resveratrol post-irradiation (RAD>RES) also resulted in significant (p 0.05) and only borderline more beneficial than continued resveratrol started two hours post-irradiation (p≈0.05) at 30 days; however, resveratrol initiated pre-irradiation was more effective (p