A method of utilizing plant growth regulator NAA induction fresh water chlorella pyrenoidosa ZF algae strain efficient accumulation carotenoid

The present invention provides a kind of method using plant growth regulator NAA induction fresh water chlorella pyrenoidosa ZF algae strain efficient accumulation carotenoid, it is characterized in that using following steps: 1) preparing algae solution: under conditions of 23 DEG C ± 2 DEG C, 1900 ± 100lx of light intensity, Light To Dark Ratio is 12h/12h, using 1/4 times of concentration BG11 nutritive salt culture frustule to logarithmic growth phase, the 1 times of concentration BG11 nutritive salt of addition in period every 14 ± 1 days, shakes algae 3 ± 1 times daily；2) it accumulates carotenoid: 200ml logarithmic growth phase algae solution being taken to be placed in 300mL conical flask, NAA to 20 ± 0.5mg/L is then added, is cultivated 14 ± 1 days under above-mentioned the same terms, inducing carotenoid Rapid Accumulation.The technology of the present invention is mature, simple and easy, low in cost, efficient pollution-free, can significantly improve chlorella pyrenoidosa ZF algae strain carotenoid output in a short time.