PP-028 Clinical and hemodynamics features of gastric varices

We report results of a study to determine the prevalence of extended-spectrum beta-lactamase (ESBL) and AmpC beta-lactamase (AmpC) producing strains among multidrug resistant (MDR) Gram-negative isolates at a teaching hospital in Nepal. Clinical isolates from patients with lower respiratory tract infection (LRTI) and urinary tract infection (UTI) were tested to detect antibiotic susceptibility, ESBL and AmpC producing following standard methods as described by American Society for Microbiology (ASM) and National Committee for Clinical Laboratory Standard (NCCLS). Among the 230 sputum samples, 30.9% (71/230) showed clinically significant isolates with 53.5% (38/71) MDR, 22.5% (16/71) ESBL producers, and 8.45% (6/71) AmpC producers. Among 1360 urine samples collected from clinically suspected UTI, 30.8% (419/1360) had significant bacterial growth with 62% (260/419) MDR, 19.1% (80/419) ESBL Producers, and 3.34% (14/419) AmpC producers. We believed the ESBL production is due to genetic coding present in the chromosomal or in plasmid DNA and AmpC production could be plasmid mediated. However, we need to subject these strains for genetic study in order to acquire genetic make up of these strains and to characterize the mechanism of drug resistance.