COMPARISON BETWEEN AMPEROMETRIC AND CHEMILUMINESCENCE METHODS IN DETECTION AND DOSIMETRY OF COBALT-60 GAMMA RAY EMISSION

Background : Hydrogen peroxide (H 2O2), produced by gamma ray irradiaon to watery soluon, was used as an analycal paramet er for dosimetry of cobalt-60 ( 60 Co) radiaon. Materials and Methods : Detecon of the produced H2O2 was carried out using two methods: an amperometric biosensor fabricated by immobilizaon of anthraquinone 2-carboxylic acid modified horseradish peroxidase on glassy carbon electrode, and chemiluminescence technique using luminol as a fluorophore and diperiodatocuprate as a catalyst. Results : In the first method, at the applied potenal of -550 mV (vs. Ag/AgCl) the biosensor showed the sensivity of 74.46 nA/Gray and detecon limit of 0.061 Gray (R.S.D=3.67% for N=3) towards H2O2 produced by