Study on Polyploid Induction of Populus yunnanensis Dode by Tissue Culture Combined with Colchicine

Populus yunnanensis Dode was employed to induce polyploid by tissue Culture combined with colchicine in order to obtain new polyploid P.yunnanensis Dode.The result showed that the better combinations were 80 mg/L colchicine treated for 30 days or 90 mg/L colchicum treated for 20-30 days during tissue culture,and gotten the highest inducing rate was 18.6%.The morphological difference,chromosome number,and stomas size were also observed between normal and various P.yunnanensis.The morphological difference was very clearer between normal and variant P.yunnanensis.The chromosome number increased to 76,and the size of stomas increased in variant P.yunnanensis.The chlorophyll content in leaves was 1.5 times higher in variant P.yunnanensis than that in normal plants.