Capillary electrophoresis determination of sulpiride enantiomers

Objective:To develop a method for chiral separation of sulpiride enantiomers and determination of the levosulpiride content in tablets by capillary electrophoresis.Method:Fused silica capillary column(50 μm×31 cm,21 cm of effective length) was adopted,and 20 mmol·L-1 citrate buffer solution containing 2% high sulfated-α-cyclodextrins was used as running buffer.The detection wavelength was set at 214 nm.The separation voltage was-10 kV.Results:Baseline separation of levosulpiride and dextrosulpiride was achieved.The linearity was excellent in the concentration ranged from 0.001-0.1 mg·mL-1 for levosulpiride.The labeled contents of levosulpiride in samples were 97.08%,98.77%,and 98.78%,respectively.Conclusion:The established method is convenient and with high sensitivity,which can be applied for quality control of sulpiride tablets.