Taxonomy characterization and cadmium biosorption of fungus strain

Abstract The taxonomy characteriazation and cadmium (Cd) biosorption of the high Cd-resistant fungus M1 were investigated. The internal transcribed spacers (ITS) region and β -tubulin genes of the strain were amplified, sequenced and analyzed by molecular biology technology. The Cd biosorption assay was performed by shaking flask. Fourier transform infrared spectroscopy was used to analyze the mycelium. The similarity of gene sequences and phylogenetic trees show the very close relation between the strain and Paecilomyces lilacinus , and the fungus M1 was identified as P. Lilacinus . The initial pH 6 and Cd concentration about 100 mg/L are optimum. Zn and Mn have a little effect on the Cd biosorption of the strain, while Cu and Pb present obvious effects. FTIR analysis shows that the fungus adsorbs Cd by esters, anhydride, and amide. With the preferable absorption capacity, fungus M1 is considered to have good prospects in bioremediation.