HPLC Fingerprint of Scrophulariae Radix from Southwest China

Objective: To establish HPLC fingerprints of Scrophulariae Radix methanol extract from both the different southwest origin and market,provide evidence for its quality control.Methods: 11 batches of samples analysis was performed on C18 column with gradient mobile phase of acetonitrile-0.4% acetic acid,the monitoring wavelength was at 260 nm with the flow rate of 1.0 mL/min,detective course was finished in 55 min.Results: Established the HPLC fingerprint common patterns by using harpagoside and cinnamic acid as the index components,and similarity with different origin rude drugs and pieces was compared.The similarity was above 0.901.Conclusion: This method contributes to the fast comprehensive evaluation of material quality and can be used specifically for standardized planting and quality control of Scrophulariae Radix.