Asymmetric interference rna compositions and uses thereof

The present invention provides sequence-specific gene silencing can be realized asymmetric duplex RNA molecule. The RNA molecule comprises a first strand and a second strand. The first strand is longer than the second strand. The RNA molecule comprising a double-stranded region formed by the first strand and a second strand, and the 5'-overhangs independently selected, two end heads peace -overhang ends. RNA molecules of the invention can be used as research tools and / or therapeutic agents.