Genome analysis of two multidrug-resistant Escherichia coli O8:H9-ST48 strains isolated from lettuce.

Abstract Vegetables may become contaminated with antibiotic-resistant bacteria from farm-to-fork. Here we report draft genome sequences of two multidrug-resistant Escherichia coli isolated from lettuce. Whole genomes of strains Y15 V.22 and Y15 V.54 were sequenced. Available tools were used to inspect for virulence factors (VF), metals tolerance, resistome and mobilome features. The predicted genome sizes were 5,4 Mb and 6,2 Mb for Y15 V.22 and Y15 V.54, respectively, both with 50.7% GC content, ST48 and serotype O8:H9. Resistome analysis showed genes encoding resistance to β-lactams, sulphonamides, trimethoprim, tetracyclines and macrolides. Cobalt, cadmium, zinc and copper tolerance determinants were identified in both. VF detected included genetic determinants related to toxin production, adherence and invasion. SNPs and VF content analysis showed a close relatedness to ETEC. Putative genomic islands, prophage and CRISPR sequences were predicted. The genome sequences here reported will aid in understanding antibiotic resistance transfer between vegetables consumed raw and humans.