Enrichment and purification of phenols from Callicarpa nudiflora Hook. et Arn by HP-20 macroporous resin

Keywords: C. nudiflora; phenols; HP-20 macroporous resin Abstract: Objective To study the technological parameters of the purification process of phenols from Callicarpa nudiflora (C. nudiflora ) with HP-20 macroporous resin.Methods The kinetic adsorption and desorption experiments were carried out on HP-20 macroporous resin (HP-20) to optimize the separation process of phenols. Additionally, the effects of four parameters including adsorption flow rate, elute flow rate, volume of 10% ethanol and 50% ethanol solution for elution were explored by a L4/3 orthogonal experiment. Finally, content of phenols in samples before and after being treated by HP-20 were compared. Results The results showed that a good separation and purification of crude total phenols extract from C. nudiflora was achieved using HP-20. The optimum conditions were found to be: initial concentration of 50 mg·mL -1 , absorb flow rate of 1 mL·min -1 , elute flow rate of 3 mL·min -1 , 5 BV of 10% ethanol solution for prewash and 10 BV of 50% ethanol solution for desorption solvent. Conclusion The content of phenols is above 40% in C. nudiflora after being treated by HP-20, indicating that HP-20 could be successfully applied to enrich and purify phenols in C. nudiflora.