cDNA cloning and prokaryotic expression of human neural RNA binding protein HuC

Objective To clone human neural RNA binding protein HuC cDNA,express and purify the recombinant human HuC(Hu antigen C)protein in E.coli.Method Human HuC cDNA was cloned by RT-PCR.HuC cDNA was inserted into the prokaryotic expression vector pGEX-4T-3.The recombinant protein HuC was expressed in E.coli BL-21,and purified by the GST Sepharose 4B affinity column.Results The(62 ku) recombinant GST-HuC fusion protein was obtained.Conclusion The recombinant human HuC protein was successfully prokaryotic expressed and purified.