Multiple turnovers of DNAzyme for amplified detection of ATP and reduced thiol in cell homogenates

A conveniently amplified DNAzyme-based fluorescence strategy was designed for highly sensitive detection of ATP or reduced thiol based on the introduction of an ATP aptamer or a disulfide bond in the bioconjugates of magnetic nanoparticles (MNP) and polystyrene microsphere–DNAzyme complexes (PSM–DNAzyme).