CHARACTERIZATIONOFAD-AMINOACIDOXIDASEMICROBIOSENSORFORD- SERINEDETECTIONINTHE CENTRAL NERVOUS SYSTEM

D-serine isa glial neuromodulator thatislinked toseveral braindisorders suchas schizophrenia, epilepsy, orstroke. We havedeveloped aninsitu D-serine microbiosensor that consists in acylindrical platinum microelectrode covered withalayer ofD-amino acidoxidase fromtheyeast R. gracilis. Thisenzymeconverts D-serine into hydroxypyruvate, while producing hydrogen peroxide that, 0 inturn, isoxidized bytheelectrode. Interferences bybiogenic amines orascorbic acid aregreatly reduced byanadditional membrane ofpoly-m-phenylene diamine. Ourmicrobiosensor canmonitor D-serine levels invivowithahigh temporal andspatial resolution. Thismethodwill makeitpossible tostudy D- o 3 serine dynamics inthecentral nervous system.