Use of polimerase chain reaction (PCR) for detection of Pseudocercosporella herpotrichoides in winter wheat cultivated in different production systems

Pseudocercosporella herpotrichoides was detected by PCR in winter wheat cultivated in different production systems in 2000 and 2001. In all tested systems only pathotype R of pathogen was detected. Detection of fungus depended on production system and weather conditions. Pathogen was not detected in both tested cultivars only in ecological system but only in season with less favourable conditions for disease development