Preparation method of duck hepatitis antibody

The invention relates to the field of immunology, in particular a preparation method of duck hepatitis antibody. The preparation method comprises the following steps: (1) feeding healthy laying hens by poplar bark lipoid at a dosage of 0.08 to 0.1g per hen each day; carrying out duck hepatitis inactivated vaccine intensive immunity for healthy laying hens fed by poplar bark lipoid after 20 to 30 days; collecting hen blood serum to measure anti-DHV neutralizing antibody titer; and collecting eggs laid by qualified flocks; and (2) separating egg white under aseptic conditions, mashing yolk and evenly mixing the yolk liquid with aseptic physiological saline to prepare yolk antibody. Compared with the antibody from mammals, antibody prepared through the preparation method has the advantages of convenient material sources, simple separation and purification method, high yield, high specificity and better stability.