Prevention of glucose toxicity in HIT-T15 cells and Zucker diabetic fatty rats by antioxidants (diabetes! insulin gene! oxidative stress! glucotoxicity)

Chronic exposure of pancreatic islets to su- praphysiologic concentrations of glucose causes adverse al- terations in ! cell function, a phenomenon termed glucose toxicity and one that may play a secondary pathogenic role in type 2 diabetes. However, no mechanism of action has been definitively identified for glucose toxicity in ! cells. To ascertain whether chronic oxidative stress might play a role, we chronically cultured the ! cell line, HIT-T15, in medium containing 11.1 mM glucose with and without the antioxi- dants, N-acetyl-L-cysteine (NAC) or aminoguanidine (AG). Addition of NAC or AG to the culture medium at least partially prevented decreases in insulin mRNA, insulin gene promoter activity, DNA binding of two important insulin promoter transcription factors (PDX-1! STF-1 and RIPE-3b1 activa- tor), insulin content, and glucose-induced insulin secretion. These findings suggested that one mechanism of glucose toxicity in the ! cell may be chronic exposure to reactive oxygen species, i.e., chronic oxidative stress. To ascertain the effects of these drugs on diabetes, NAC or AG was given to Zucker diabetic fatty rats, a laboratory model of type 2 diabetes, from 6 through 12 weeks of age. Both drugs pre- vented a rise in blood oxidative stress markers (8-hydroxy- 2! -deoxyguanosine and malondialdehyde " 4-hydroxy-2- nonenal), and partially prevented hyperglycemia, glucose intolerance, defective insulin secretion as well as decrements in ! cell insulin content, insulin gene expression, and PDX-1 (STF-1) binding to the insulin gene promoter. We conclude that chronic oxidative stress may play a role in glucose toxicity, which in turn may worsen the severity of type 2 diabetes.