Effects of Agar and Liquid Media on Growth Behaviors of Zoysiagrass In Vitro

In vitro shoot propagation from shoot tip explants of zoysiagrass (Zoysia matrella Merr.) was achieved in agar and liquid media based on Murashige and Skoog (1962) media supplemented with various combinations of α-naphthaleneacetic acid (NAA) and 6-benzyl-aminopurine (BA) concentrations. Maximum shoot propagation was obtained with 0.5μM NAA and 5.0μM BA in agar media, and 0.05μM NAA and 0.5μM BA in liquid media. Stolons were formed in liquid media but not in agar media. Liquid media was superior for propagation of shoots to agar media as the formations of multiple shoots, stolons and roots were higher in rates.