魚類筋肉からのミオシンのnative thick filamentの調製と性状

Myosin native thick filamant(nTF)was prepared as usual from the muscle of carp, tilapia and rabbit for reference. The three nTF's were comparable to each other in both length and Polarity. Reformed thick filament (rTF) which was prepared from each fish nTF by dissolvingin 0.5MKCl and diluting was definitely shorter than the original nTF, in contrast to rabbit nTF, which showed essentially the same length as the nTF. Carp and rabbit rTF's were bipolar, whereas tilapia rTF was not. However, tilapia nTF gave rise to a bipolar rTF when prepared in the presence of more than s mM MgClz. Fish rTF of bipolar nature exhibited essentially the same actin-activated Mg-ATPase acrivity as did their nTF's.