Preparation of Monoclonal Antibodies against Equine Influenza Virus Subtype H7N7

Objective To prepare the monoclonal antibodies(mAbs) against equine influenza virus(EIV) subtype H7N7 and develop a specific,sensitive and simple method for determination of the virus.Methods BALB / c mice were immunized with EIV subtype H7N7,of which splenocytes were fused with myeloma SP2 / 0 cells.The hybridoma cell strains stably secreting the mAbs against EIV subtype H7N7 were screened by hemagglutination inhibition(HI) test and indirect ELISA,and the biological characteristics of the secreted mAbs were identified.Results Three hybridoma cell strains stably secreting mAbs against EIV subtype H7N7 were screened,named as 5B2,1C10 and 2B7 respectively.The mAbs secreted by 5B2 and 1C10 cell strains were IgG2a,while that by 2B7 cell strain was IgG M,of which all the light chains were κ chains.All the mAbs reacted specifically with EIV subtype H7N7,while showed no cross reaction with EIV subtype H3N8,equine arteritis virus(EAV),equine infectious anemia virus(EIAV) or equine encephalitis virus(JEV),indicating high specificity.Conclusion Three kinds of mAbs specific to EIV subtype H7N7 were prepared,which provided good materials for rapid diagnosis and epidemiological investigation of EIV subtype H7N7.