SRAP Molecular Marker in the WSSV Resistance of Fenneropenaeus chinensis Named "Huanghai No.1"

The SRAP(sequence related amplified polymorphism) molecular marker was used to screen the specific sequence of WSSV(white spot syndrome virus) resistance in Fenneropenaeus chinensis.The research object F.chinensis was divided into WSSV susceptible group and WSSV resistant group,and SRAP was performed with 52 pairs of primers with good amplified polymorphism.After analyzing the results with bioinformatic methods,11 specific sequences were considered to be possilly related to WSSV resistance in F.chinensis.Then the specific sequences were compared with sequences from NCBI.The comparison showed that the marked sequences of number 40.3,4.20,25.14 had 93 %,100 %,87 % sequence identity with EST of salmon(Salmo salar) heavy metal efflux system protein,EST of sheep(Ovis aries) immunoglobulin,and EST of channel catfish(Ictalrus punctatus) aldehyde dehydrogenase,respectively.The marked specific sequences were deduced to participate the expression of corresponding functional proteins,which may not only enhance the WSSV resistance in F.chinensis indirectly,but also be used as molecular markers for WSSV resistance screen in F.chinensis breed selection.