High-throughput analysis of population quality of Huanglian Shangqing Pills by near infrared spectroscopy

AIM To establish high-throughput,rapid and non-destructive approaches of near infrared spectro scopy to analyse the population quality variances of Huanglian Shangqing Pills. METHODS Concentrated and watered pills and common watered pills of Huanglian Shangqing Pills were obtained from six manufacturers and served as calibration set and validation set,respectively. Near infrared diffuse reflectance spectra combined with principle component analysis were used for discriminant analysis and the calculation of covariance matrix S for generalized variance analysis. RESULTS The accuracy of predicting model,calibration set and validation set were approximately to 100%. CONCLUSION Near infrared spectroscopy plus discriminant analysis could distinguish the manufacturers and their preparations,and plus generalized variance would present the population quality of Huanglian Shangqing Pills.