Distribution of MN genotypes detected by PCR-SSCP analysis

Abstract Genotyping of the MN blood system was performed by means of PCR-single strand conformation polymorphism (PCR-SSCP) analysis. Twelve band patterns corresponding to each MN genotype composed of alleles M G , M T , N 1 , N 2 and N V were detected. In general, M G or N 1 >M T >N 2 in order of decreasing frequency was observed as four common alleles in five Japanese, two Chinese and a German populations. PCR-SSCP analysis provides more discriminative classification to the MN genotyping.