Evaluation of cerium oxide and cerium oxide based fuel additive safety on organotypic cultures of lung slices

Among the various technological proposals under consideration to limit diesel exhaust particles (DEP) emissions, the use of a particulate filter in conjunction with a fuel-borne catalyst is currently the primary focus and likely to be introduced over the coming years. One specific catalyst is a cerium-based compound Envirox™ developed by Oxonica (Kidlington, UK) and used as a fuel additive to reduce particulate matter (PM) emissions from diesel engines. Prior to any commercialization it is necessary to undertake an assessment of any potential adverse health effects resulting from exposure to cerium oxide particles especially via the inhalation route, i.e., on the lung. Since the composition of engine exhaust emissions from additized fuel might be different to those emissions from un-additized diesel fuel, the second phase of the current investigation compared the potential biological impact of engine emissions from cerium oxide additized fuel to that of a reference fuel. An experimental procedure develope...