Oral administration of CTB insulin B chain produced in silkworm delays the diabetes development in NOD mice

An fusion protein that insulin B-chain peptide(InsB)was conjugated with the cholera toxin B subunit(CTB) was administered orally to NOD mice which could develop type Ⅰ diabetes spontaneously,in order to demonstrate the diabetes delay effect of CTB-InsB.The urine glucose then was observed,the blood glucose was detected and the effect of the fusion protein was analyzed.Compared with the control group which was fed with wild type silkworm blood lymph,this fusion protein at the dosage of 50 μg per feeding can statistically efficaciously prevents the mice from the rapid development of diabetes(P0.05).Whereas the administration of 100 μg and 10 μg per feeding appears not so distinctly to decrease the incidence of diabetes (P0.05),although those dosages still show downtrend.