Dextranase の動物の皮膚および粘膜におよぼす影響 (第3報)

The irritation property of dextranase which was derived from Chaetomium gracile was determined to the rabbit skin and eye, and to the hamster cheek pouch membrane.1) Rabbit skin; Cotton cloths containing 300000 units/0.5ml of dextranase were patched on intact or abraded skin of the rabbit. After 24 and 72 hours, the irritancy of dextranase to intact or abraded skin were almost the same. After 24 hours only one out of three animals indicated slight erythema, and the irritation index was 0.2.2) Rabbit eye; On the rabbit eye 60000 units/0.1ml of dextranase was dropped once. After 1, 24, 48, 72 hours and 7 days, the irritancy of dextranase was observed according to the Draize's method. After 1 hour only one out of three animals indicated slight hyperemia of conjunctivae, and the mean total irritation score was 0.3.3) Hamster cheek pouch membrane; During 7 days, 1200000 units of dextranase was administered once a day in hamster cheek pouch by means of liquid or powder. However, any significant change could not be observed by the naked eyes or microscopic observations.These results suggested that the irritation property of dextranase to skin, eye and oral mucosa may be very slight or none. In particular, 20000 units/g of dextranase in the tooth paste may be out of question in its regular use.