Label-free tracking of whole-cell response on RGD functionalized surfaces to varied flow velocities generated by fluidic rotation

Abstract Fluidic flow plays important roles in colloid and interface sciences. Measuring adsorption, aggregation processes and living cell behavior under a fluidic environment with varied flow velocities in a parallel and high-throughput manner remains to be a challenging task. Here we introduced a method to monitor cell response to well-defined flow with varied velocities over an array of label-free resonant waveguide grating (RWG) based optical biosensors. The arrangement consists of a circular well with an array of biosensors at the bottom surface. By rotating the liquid over the biosensor array using a magnetic stirrer bar, flow velocities from zero to a predefined maximum can be easily established over different locations within the biosensor array as characterized in detail by numerical simulations. Cell adhesion and detachment measurements on an Arg-Gly-Asp (RGD) peptide functionalized surface were performed to demonstrate i) measurements at a wide range of simultaneous flow velocities over the same interface; ii) the possibility of parallel measurements at the same flow conditions in one run; and iii) the simple tuning of the employed range of flow velocities. Our setup made it possible to analyze the magnitude and rate of cell detachment at various flow velocities in parallel and determine the critical velocity and force where cells start to detach from the RGD motif displaying biomimetic surface. Furthermore, cellular response to simultaneous mechanical (flow) and chemical stimulation was also investigated using trypsin as a model. This study opens a new possibility to investigate interface phenomena under predefined and conveniently varied flow conditions.