Mise en évidence par chromatofocalisation des isoenzymes de la fucosyl-transférase cérébrale d'agneau

Summary Glycoprotein : fucosyltransferase from sheep brain has previously been solubilized with detergent Triton X 100 and prepurified by hydrophobic chromatography on ethyl-agarose. Additional purification was performed using a new method, namely chromatofocalisation. With a small column (30 ml) of P B E 94 Pharmacia eluted by a mixture of polybuffers 76 and 94, we were able to separate quickly four isoenzymes of the cerebral fucosyltransferase in the pH range 5–8. With regard to speed and ease, this technique seems to be very useful in purification of glycosyltransferases.