Characterization of Galacto-oligosaccharides using High-Performance anion exchange Chromatography-tandem mass spectrometry.
2021
The analysis of complex oligosaccharide mixture remains a challenge in the field of analytical chemistry. In this work, two commercial galacto-oligosaccharides samples were characterized using high-performance anion exchange chromatography coupled to mass spectrometry. The isomeric oligosaccharides were resolved with high resolution. The structures of the individual isomers with DP up to 6 were analyzed using targeted selected ion monitoring with data-dependent tandem mass spectrometry, with additional in-source collision-induced dissociation. This article is protected by copyright. All rights reserved.
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